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KMID : 1024420070110040305
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Food Engineering Progress
2007 Volume.11 No. 4 p.305 ~ p.312
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Estrogenic activity and Physiological Active Components of Iranian Black Pomegranate Seed Extracts
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Kim Hyuk-Hwa
Koh Jong-Ho
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Abstract
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This study was investigated on estrogenic activities and physiological active components of water extracts, ethanol extracts and oil of pomegranate seed. The extraction yields of water extract(PE-HW), 50%ethanol extract(PE-ET50), 80%ethanol extract(PE-ET80), 95%ethanol extract(PE-ET95), and fine oil of pomegranate seed were 28.5%, 14.5%, 13.2%, 12.3%, and 4.0%, respectively. The total ellagic acid contents of PE-HW, PE-ET50, PE-ET80 and PE-ET95 analyzed by HPLC were 195.0, 486.0, 399.4 and 251.4 μg/g. After hydrolytic treatment by hydrochloride, total ellagic acid contents of acidified PE-HW, acidified PE-ET50, acidified PE-ET80, and acidified PE-ET95 were 550.0, 1474.1, 1166.8, and 694.1 μg/g, respectively. In estrogenic evaluation by β-galactosidase assay, 17β-estradiol(E2) showed strong activity at 10-9M, whereas all extracts and oil of pomegranate seed showed no significant results at 1.00 mg/ml. Daidzein and genestein was not detected in seed.
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KEYWORD
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pomegranate seed, estrogenic activity, ellagic acid, punicic acid
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